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SPECHT Alexandre

Chargé de Recherche - CNRS

Contact : specht@unistra.fr

Research Theme

- caged compounds
- Photoregulation of Ligand Gated Ion Channels.

Functions

  • since october 2008 : Chargé de Recherche 1er classe, CNRS.
  • 2004-2008 : Chargé de Recherche 2ème classe, CNRS.
  • 2003 : Research Associate au Howard Huges Medical Institute / UCLA (Los Angeles).
  • 2002 : Attaché Temporaire d’Enseignement et de Recherche (Strasbourg I).
  • 1999-2002 : PhD fellowship B.D.I. CNRS / Région Alsace.

Selected Publications

Characterization of one- and two-photon photochemical uncaging efficiency. A. Specht, F. Bolze,JF. Nicoud, M. Goeldner, Methods Mol Biol., 2013 ;995:79-87.

Ligand-Gated Ion Channels : New Advances in Neurological Disorders and Ligand Recognition D. Lemoine, R. Jiang , A. Taly, T. Chataigneau, A. Specht, T. Grutter, Chem. Rev., 2012, 112 (12), pp 6285–6318

Intermediate closed channel state(s) precede(s) activation in the ATP-gated P2X2 receptor. R. Jiang , A. Taly, D. Lemoine, A. Martz, A. Specht, T. Grutter, Channels, 2012, 6(5), 1-5.

Two-photon uncaging : The chemist point of view. F. Bolze, J-F. Nicoud, C. Bourgogne, S. Gug, X.H. Sun, M. Goeldner, A. Specht, L. Donato, D. Warther, G.F. Turi, A. Losonczy, Optical Materials, 2012, 34 : 1664-1669.

The donor-acceptor biphenyl platform : A versatile chromophore for the engineering of highly efficient two-photon sensitive photoremovable protecting groups. A. Specht*, F. Bolze, L. Donato, C. Herbivo, S. Charon, D. Warther, S. Gug, J-F. Nicoud, M. Goeldner Photochem Photobiol Sci, 2012, 11(3) : 578-586.

New water-soluble, donor-acceptor biphenyl derivatives in the 2-(o-nitrophenyl)-propyl series for highly efficient two-photon uncaging of the neurotransmitter GABA at 800 nm. L. Donato, A. Mourot, C. M. Davenport, C. Herbivo, D. Warther, J. Léonard, F. Bolze, J-F. Nicoud, R. H. Kramer, M. Goeldner, A. Specht*, Angew. Chem. Int. Ed., 2012, 51(6) : 1840-1843.

Two-photon excitation in life sciences : neurotransmitter and fluorescence uncaging F. Bolze, J-F. Nicoud, S. Gug, S. Charon, A. Specht, M. Goeldner, D. Warther, X-H. Sun, P. Kessler, Y. Lutz, J-L. Vonesch and A. Losonczy, Proc. SPIE , 2011, 7903, 790339

Time-Resolved WAXS Reveals Accelerated Conformational Changes in Iodoretinal-Substituted Proteorhodopsin. E. Malmerberg, Z. Omran, J. S. Hub, X. Li, G. Katona, S. Westenhoff, L. C. Johansson, M. Andersson, M. Cammarata, M. Wulff, D. van der Spoel, J. Davidsson, A. Specht, R. Neutze, Biophysical Journal, 2011, 101 : 1345–1353.

Agonist trapped in ATP-binding sites of P2X2 receptor. R. Jiang , D. Lemoine, A. Martz, A. Taly, S. Gonin, L. Prado de Carvalho, A. Specht, T. Grutter, Proc. Natl. Acad. Sci. USA, 2011, 108(22) : 9066-9071

Retrochalcone derivatives are positive allosteric modulators at synaptic and extrasynaptic GABAA receptors in vitro.R. Jiang, A. Miyamoto, A. Martz, A. Specht, H. Ishibashi, M. Kueny-Stotz, S. Chassaing, R. Brouillard, L. Prado de Carvalho, M. Goeldner, J. Nabekura, M. Nielsen, T. Grutter, British journal of Pharmacology, 2011, 162 (6), 1326-1339 .

Small photoactivatable molecules for controlled fluorescence activation in living cells, D. Puliti , D. Warther, C. Orange, A. Specht, M. Goeldner, Bioorg. Med. Chem., 2011, 19 (3), 1023-1029

Two-photon uncaging : new prospects in neuroscience and cellular biology.”, D. Warther, S. Gug, A. Specht, F. Bolze, J-F. Nicoud, A. Mourot, M. Goeldner, Bioorg. Med. Chem., 2010, 18(22):7753−7758.

Live-cell One- and Two-Photon Uncaging of a Far-Red Emitting Acridinone Fluorophore », D. Warther, F. Bolze, J. Léonard, S. Gug, A. Specht, D. Puliti, X.-H. Sun, P. Kessler, Y. Lutz, J-L. Vonesch, B. Winsor, J-F. Nicoud, M. Goeldner, J. Am. Chem. Soc., 2010, 132, 2585-2590.

Two−Photon Excitation in Life Sciences : From Observation to Action, F. Bolze, A. Niehl, M. Heinlein, N. Mjdasiri, J.-L. Rehspringer, N. Schaeffer, P. Didier, Y. Arntz, Y. Mély, S. Gug, A. Specht, M. Goeldner, J.-F. Nicoud, Nonlinear Optics Quantum Optics, 2010, 40 (1−4), 253−265

Synthesis and photochemical properties of new photo-cleavable crosslinkers for the triggering of biological responses Z. Omran, A. Specht*, Tetrahedron Lett., 2009, 50, 2434_2436.

Photochemical tools to study dynamic biological processes ». A. Specht*, F. Boltze, Z. Omran, J−F. Nicoud, M. Goeldner, HFSP Journal, 2009, 3(4), 255−264

Complexing photolabile cholinergic ligands with synthetic and biological receptors : a dynamic survey. A. Specht, S. Loudwig, L. Peng, M. Goeldner, Journal of Inclusion Phenomena, 2009, 65, 157–163.

Short−length dimethoxynitrophenyl photo−cleavable crosslinkers,synthesis and photolysis, Z. Omran, A. Specht, Journal of Photochemistry and Photobiology A : Chemistry, 2009, 208, 125–130

Phototriggering cell adhesion by caged cyclic RGD peptides., A. Petersen, J. M. Alonso, A. Specht, P. Duodu, M. Goeldner, A. del Campo, Angew. Chem. Int. Ed., 2008, 3192-3195.

Synthesis and Photochemical Properties of a Light-activated Fluorophore to label His-tagged-Proteins C. Orange, A. Specht*, D. Puliti, Elias Sakr, T. Furuta, B. Winsor and M. Goeldner, Chem. Commun., 2008, 1217-1219.

Glutamate photolabile protecting groups with high one and two-photon uncaging efficiencies., S. Gug, S. Charon, A. Specht, K. Alarcon, D. Ogden, B. Zietz, J. Leonard, S. Haacke, F. Bolze, J-F. Nicoud and M. Goeldner, ChemBioChem, 2008, 1303-1307.   The Crystal Structure of a Sodium Galactose transporter Reveals Mechanistic Insights into Na+ / Sugar Synport. S. Faham, A. Watanabe, G. M. Besserer, D. Cascio, A. Specht, B. A. Hirayama, E. M. Wright, J. Abramson., Science, 2008, 321 : 810-814.

Comparative models of P2X2 receptor support inter-subunit ATP-binding sites G. Guerlet, A. Taly, L. Prado de Carvalho, A. Martz, R. Jiang, A. Specht, N. Le Novère T. Grutter, Biochemical and Biophysical Research Communications, 2008, 375, 405-409.

Molecular engineering of photoremovable protecting groups for two photon uncaging S. Gug, F. Bolze, A. Specht, C. Bourgogne, M. Goeldner, J-F. Nicoud, Angew. Chem. Int. Ed., 2008, 3192-3195.

Use of a ‘caged’ analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography, J.-P. Colletier, A. Royant, A. Specht, B. Sanson, F. Nachon, P. Masson, G. Zaccai, J. L. Sussman, M. Goeldner, I. Silman, D. Bourgeois and M.Weik, Acta Crystallogr. D Biol. Crystallogr., 2007, 1115-1128.

New Photoremovable Protecting Groups for Carboxylic Acids with High Photolytic Efficiencies at Near-UV Irradiation. Application to the Photocontrolled Release of L-Glutamate. A. Specht, J-S. Thomann, K. Alarcon, W. Wittayanan, D. Ogden, T. Furuta, Y.Kurakawa and M. Goeldner, ChemBioChem., 2006, 1690-1695.

p-Sulfonated calixarenes as synthetic receptors to complex photolabile cholinergic ligands. A. Specht, F. Ziarelli, M. Goeldner, L. Peng ; Helv. Chem. Acta, 2005, 2641-2653.

1-(o-Nitrophenyl)-[2,2,2]-trifluoroethyl-ether derivatives as stable and efficient photoremovable alcohol protecting groups : potential use for the caging of alcohol–containing biomolecules. , A. Specht and M. Goeldner ; Angew. Chem. Int. Ed., 2004 2008-2012.